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Widal (TO, TH, AH, BH)

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Description

Product Information: Widal Test (TO, TH, AH, BH)

The Widal Test is a diagnostic serological test used to detect antibodies against Salmonella bacteria, which cause typhoid and paratyphoid fever. This test is commonly employed to confirm suspected cases of typhoid and assess immunity levels in individuals.

Key Features:

  • Antigens:
    • TO (Somatic antigen)
    • TH (Flagellar antigen of Salmonella typhi)
    • AH (Flagellar antigen of Salmonella paratyphi A)
    • BH (Flagellar antigen of Salmonella paratyphi B)
  • Type: Qualitative and semi-quantitative agglutination test.
  • Sample Required: Serum or plasma.
  • Specificity: Designed to detect both IgM and IgG antibodies against Salmonella antigens.
  • Packaging: Supplied with antigen vials, positive controls, and detailed instructions.

Intended Use:

  • Diagnosis of typhoid and paratyphoid fever.
  • Monitoring immunity or exposure to Salmonella infections.
  • Screening in endemic areas or post-vaccination antibody response.

Advantages:

  • Rapid and reliable results for typhoid diagnosis.
  • High-quality antigen reagents for accurate antibody detection.
  • Cost-effective and easy-to-use in standard clinical laboratories.

Testing Procedure:

  1. Mix the patient’s serum or plasma with specific antigens (TO, TH, AH, BH).
  2. Observe for agglutination patterns under appropriate conditions.
  3. Compare results against controls to interpret antibody titers.

Storage and Stability:

  • Store antigens at 2–8°C. Avoid freezing.
  • Stable under recommended conditions until the expiration date on the label.

Precautions:

  • This test should be conducted by trained professionals.
  • Results should be interpreted alongside clinical findings and other diagnostic procedures.
  • False positives/negatives may occur; confirmatory tests may be required.

Applications:

  • Clinical settings for the diagnosis of enteric fevers.
  • Epidemiological surveys in typhoid-prone regions.
  • Academic research for studying Salmonella immunology.

Manufacturer Support:

Technical assistance and product guidance are available upon request. Contact us for additional resources or troubleshooting.

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