TB-IgG Elisa Kit

Description

Product Information: EV71-IgM ELISA Kit

Product Name: EV71-IgM ELISA Kit
Product Code: [Insert Product Code]
Brand: [Insert Brand Name]
Format: 96-well microplate
Detection Method: Enzyme-Linked Immunosorbent Assay (ELISA)

Description:
The EV71-IgM ELISA Kit is a highly sensitive and specific diagnostic tool designed for the detection of Immunoglobulin M (IgM) antibodies against Enterovirus 71 (EV71) in human serum or plasma samples. This kit is used to aid in the diagnosis of EV71 infections, which are associated with hand, foot, and mouth disease (HFMD) and, in some cases, severe neurological complications.

Key Features:

• High Sensitivity & Specificity: The kit provides reliable and accurate results for detecting IgM antibodies specific to EV71.
• Rapid Results: Results can be obtained in as little as 2-3 hours.
• User-friendly: Simple and easy-to-follow protocol, suitable for both clinical and research laboratories.
• Wide Range of Applications: Ideal for screening and surveillance in clinical diagnostics and epidemiological studies.
• No Cross-Reactivity: Minimal interference from other enteroviruses, ensuring accurate results for EV71 detection.

Components Included:

1. Pre-coated ELISA Microplate (96-well format): Ready-to-use wells coated with EV71 antigens.
2. Positive Control: For validation of assay performance.
3. Negative Control: For ensuring specificity of the assay.
4. Sample Diluent: For preparing patient samples.
5. Conjugate (Anti-Human IgM HRP): Enzyme-conjugated secondary antibody for detection.
6. Substrate Solution: For visual detection of the reaction.
7. Stop Solution: To terminate the reaction and stabilize the results.
8. Wash Buffer: To remove unbound substances from the wells.

Applications:

• Detection of EV71-specific IgM antibodies in suspected cases of EV71 infection.
• Diagnostic tool for early detection of EV71 infections, especially during outbreaks of HFMD.
• Aid in epidemiological surveillance studies to assess the prevalence of EV71 in populations.

Technical Specifications:

• Detection Range: 0.1 – 100 U/mL of IgM antibodies
• Sensitivity: 98%
• Specificity: 99%
• Sample Volume Required: 100 µL per well
• Storage: Store at 2-8°C. Do not freeze components.

Instructions for Use:

1. Sample Preparation: Dilute serum or plasma samples as per the instructions.
2. Assay Procedure: Add the sample, positive control, or negative control to the appropriate wells on the microplate. Incubate according to the protocol.
3. Washing & Detection: After incubation, wash the plate and add the conjugate, followed by the substrate solution.
4. Reading the Results: Add the stop solution and measure the absorbance using a microplate reader at the recommended wavelength (450 nm).

Results Interpretation:
The presence of a color change indicates a positive result for IgM antibodies against EV71. Quantitative results can be determined by comparing the sample’s optical density (OD) to a standard curve.

Safety Information:

• For laboratory use only.
• Follow standard biosafety procedures when handling clinical samples.
• Dispose of used materials according to local regulations.

Regulatory Compliance:
This kit complies with international quality standards and is CE marked for in vitro diagnostic use (where applicable).

Shelf Life:
12 months from the date of manufacture (refer to the product packaging for the expiration date).

Packaging Options:

• 96-well microplate (for 96 tests)

Contact Information:
For more details, technical support, or bulk orders, please contact:
[Insert Contact Information]
[Insert Manufacturer’s Website]

Note:
Please refer to the detailed product insert for full instructions on usage, troubleshooting, and interpretation of results.