Estradiol Elisa Kit

Description

Product Name: Estradiol ELISA Kit

Product Code: [Insert Product Code]

Brand: [Insert Brand Name]

Product Description: The Estradiol ELISA Kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) designed to measure estradiol (E2) levels in human serum, plasma, or other biological fluids. Estradiol is a primary form of estrogen hormone, critical in regulating reproductive health, menstrual cycles, and various physiological processes in both women and men. This kit provides accurate and reliable results for research, clinical diagnostics, and hormone therapy monitoring.

Key Features:

• High Sensitivity: The kit is designed to detect low levels of estradiol with exceptional precision.
• Wide Dynamic Range: Suitable for a variety of sample types, including serum, plasma, and urine.
• Fast and Easy: The user-friendly protocol ensures quick and straightforward testing.
• Reliable Results: Provides reproducible and accurate measurements of estradiol concentration.
• Versatile Application: Ideal for clinical laboratories, research institutions, and pharmaceutical studies.

Principle of the Test: The Estradiol ELISA Kit works based on a competitive binding assay. During the assay process, estradiol present in the sample competes with a biotinylated estradiol conjugate for binding to the specific antibody coated on the microplate. The amount of conjugate bound to the antibody is inversely proportional to the concentration of estradiol in the sample. The detection is done through a colorimetric reaction, providing a measurable output corresponding to estradiol concentration.

Applications:

• Hormone level testing in clinical diagnostics.
• Monitoring estradiol levels during fertility treatments or hormone therapy.
• Research into estrogen-related disorders, such as polycystic ovary syndrome (PCOS) and menopause.
• Endocrine function evaluation in both genders.

Kit Contents:

• Pre-coated microplate (12 strips, 8 wells per strip).
• Estradiol standards (different concentrations).
• Enzyme-conjugate (horseradish peroxidase conjugated estradiol).
• Substrate solution (TMB substrate).
• Stop solution (sulfuric acid).
• Wash buffer and sample diluent.

Storage and Stability:

• Store the unopened kit at 2–8°C.
• Once opened, the reagents are stable for up to [X] months if stored properly.
• Ensure the microplate is tightly sealed to avoid contamination or moisture exposure.

Test Sensitivity:

• The assay has a detection limit of [X] pg/mL.
• The assay provides reliable results across a wide range of estradiol concentrations.

Limitations:

• For in vitro diagnostic use only.
• Results may be affected by sample storage conditions and handling.
• This assay is not suitable for use with samples from animals or non-human sources.

Instructions for Use:

1. Prepare the reagents and standards.
2. Add sample and standards to the pre-coated wells.
3. Add the enzyme-conjugate, incubate, and perform the wash steps.
4. Add the substrate solution and wait for the reaction to develop.
5. Stop the reaction and measure the absorbance at [X] nm.
6. Use the standard curve to calculate estradiol concentrations in the samples.

Specifications:

• Sensitivity: [X] pg/mL
• Assay Time: [X] minutes
• Sample Type: Serum, plasma, urine
• Detection Method: Colorimetric (OD at [X] nm)

Packaging Options:

• Tests per kit
• Available in [X]-unit boxes

Certifications:

• CE marked for in vitro diagnostic use.
• Complies with [Insert relevant standard or regulation].

For Inquiries: For additional product information, technical support, or bulk orders, please contact [Insert Contact Information].